Journal: PLoS ONE

Article Title: Virotherapy Using Myxoma Virus Prevents Lethal Graft-versus-Host Disease following Xeno-Transplantation with Primary Human Hematopoietic Stem Cells

doi: 10.1371/journal.pone.0043298

Figure Lengend Snippet: To determine if MYXV can infect CD3 + lymphocytes found in human HCT samples, 1×10 6 whole BM cells ( A ) or 1×10 6 human PBMCs ( B ) were treated with vMyx-GFP at MOI = 10. Twenty-four hours after MYXV exposure, cells were stained with antibodies against CD3 and CD4 and the levels of GFP + cells in each population was determined using flow cytometry.

Article Snippet: Fresh normal human bone marrow aspirate cells and peripheral blood mononuclear cells were obtained commercially from Lonza (Walkersville, Maryland).

Techniques: Staining, Flow Cytometry

Journal: PLoS ONE

Article Title: Virotherapy Using Myxoma Virus Prevents Lethal Graft-versus-Host Disease following Xeno-Transplantation with Primary Human Hematopoietic Stem Cells

doi: 10.1371/journal.pone.0043298

Figure Lengend Snippet: NSG mice were sublethally irradiated and then transplanted with either 5×10 6 primary human PBMCs (n = 6) or 5×10 6 primary human PBMCs pre-treated with MYXV (n = 6). Mice were weighed twice per week to monitor body condition ( A ) and sacrificed either seven weeks after transplant or when their body condition score measured 2 ( B ). Significant differences in survival were determined using the log-rank test (P<0.05). N.S. = not significant. Post-mortem, organs were extracted, fixed in formalin, sectioned and stained for the presence of human CD3 + lymphocytes ( C ). Immunohistochemistry images shown are representative of results observed in three separate mice from the three engrafted cohorts stained for the presence of human CD3 + cells.

Article Snippet: Fresh normal human bone marrow aspirate cells and peripheral blood mononuclear cells were obtained commercially from Lonza (Walkersville, Maryland).

Techniques: Irradiation, Staining, Immunohistochemistry

Journal: Biomaterials science

Article Title: Cell-secreted extracellular matrix influences cellular composition sequestered from unprocessed bone marrow aspirate for osteogenic grafts

doi: 10.1039/c8bm01478g

Figure Lengend Snippet: (A) Control media or media containing equal quantities of collagen or cell-secreted extracellular matrix (ECM) were mixed with (B) mononucleated cells from BMA and injected into (C) perfusion bioreactors containing HA-PLG scaffolds press-fit into the perfusion chamber. After 20 hours of oscillating perfusion, scaffolds were retrieved and (D) implanted into subcutaneous pockets in the back of nude rats.

Article Snippet: Seeding bone marrow aspirate in perfusion bioreactors Unprocessed human bone marrow aspirate (BMA) was from Lonza (10 mL of aspirate from n = 2 male and n = 2 female donors; 23–32 years old, mean age 26 ± 4 years).

Techniques: Control, Injection

Journal: Biomaterials science

Article Title: Cell-secreted extracellular matrix influences cellular composition sequestered from unprocessed bone marrow aspirate for osteogenic grafts

doi: 10.1039/c8bm01478g

Figure Lengend Snippet: (A) Immunophenotype of unprocessed BMA and cells entrapped in scaffolds. (B – E) Percentage of entrapped cells that are (B) hematopoietic cells (CD45+), (C) hematopoietic progenitor cells (CD45+CD34+), (D) endothelial cells (CD31+CD45−), and (E) mesenchymal stem cells (CD73+CD90+CD45−) shown as a fold change to the percentage found in fresh BMA (n=4 replicates from 1 biological donor).

Article Snippet: Seeding bone marrow aspirate in perfusion bioreactors Unprocessed human bone marrow aspirate (BMA) was from Lonza (10 mL of aspirate from n = 2 male and n = 2 female donors; 23–32 years old, mean age 26 ± 4 years).

Techniques: